We use onsrud bits. The part numbers I would use are as follows.
1/16 diameter with 1/8 shank= 64-000
1/8 diameter with 1/4 shank= 64-012
I would set the router speed to 35,000 rpm. I would also leave the
paper or plastic sheeting on the acrylic and only route through the acrylic not the
paper or plastic sheeting. This will help with them being sucked into the machine or moving. The part numbers above are downcut bits which should help improve the small pieces from moving around. Just to feel confident I would even do 2 passes. One thing I have also found is that when you are setting up the file in "Touch and Cut/Zund Cut Center" the "UP" position needs to be at least an inch off of the material. This will prevent the letter from being sucked up with the vacuum. If all else fails then I would route these with the collection vacuum turned off.