yup, the ink layers is in your print settings in your rip que. usually wherever you dial-up the temps.
make sure you allow for excess amounts of drytime as well, especially if you intend to laminate.
two layers of printed graphics is the way to go, for day/night similiarity, but if you gotta double-strike to save on $$$ then more power to ya. image is gonna be extremely dark when not lit, but its up to you if that matters at all.